Researchers analyzing the SIRT3 gene sequence engineer a SIRT3 gene that is identical to the normal gene except that a single nucleotide has been deleted from the 5′ end of the second exon of the gene; the deletion does not affect splicing. The researchers introduce this gene into normal stomach cells to determine how the primary AND tertiary structures of the protein expressed from the engineered SIRT3 gene will compare with the protein expressed from the normal SIRT3 gene in these cells. Predict the results of this experiment. The researchers claim that the data in Figure 3 provide evidence that the stomach cancer cells perform fermentation to a greater extent than do normal stomach cells. Provide reasoning to justify the claim. Based on the information provided, predict the effect on the ratio of NADH/NAD+ in the cells treated with the compound that blocks the electron transport chain compared with similar cells not treated with the compound.
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